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KMID : 0359319990390040765
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Korean Journal of Veterinary Research
1999 Volume.39 No. 4 p.765 ~ p.771
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Modified sorbitol MacConkey agar for the rapid isolation of Escherichia coli O157 : H7
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Jung, Byeong Yeal
Jung, Suk Chan/Cho, Dong Hee/Lee, Na Kyung/Cho, Seong Kun/Her, Moon/Yoon, Yong Dhuk
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Abstract
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Unlike most Escherichia coli strains, E coli O157 : H7 didn´t ferment sorbitol within 24h of incubation and showed a negative reaction for β-glucuronidase. We developed a new medium for the rapid isolation of E coli O157 : H7 using sorbitol MacConkey agar with cefixime, potassium tellurite and 4-methylumbelliferyl-β-D-glucuronide (MUG) as a primary plating medium. The addition of 20㎍/㎖ of vancomycin in enrichment broth for E coli O157 : H7 inhibited lots of Gram positive bacteria. Three strains (10.3%) of 29 non-O157 E coli strains and 3 strains (8.3%) of 36 Salmonella spp were inhibited at the 0.05㎍/㎖ of cefixime and 23 strains (79.3%) of 29 non-O157 E coli strains and 12 strains (33.3%) of 36 Salmonella spp were inhibited at the 2.0㎍/㎖ of potassium tellurite. But none of the E coli O157 : H7 was affected at these concentration. The addition of MUG at 100㎍/㎖ level to sorbitol MacConkey agar with cefixime and potassium tellurite (CTM-SMAC) aided in the rapid isolation of E coli O157 : H7 from samples by checking sorbitol-negative and β-glucuronidase negative phenotypes simultaneously. In conclusion, inoculation of a positive in the O157 screening test from enrichment broth on CTM-SMAC appeared to be a rapid, cost-effective and sensitive method for the isolation of E coli O157 : H7.
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